This paper presents a surface plasmon resonance (SPR) imaging system based on angular modulation (AM) and intensity measurement (IM) together to avoid the mechanical errors of the angle scanning device. The SPR resonant angle was found by angular scanning method and then the light intensity changes were collected at a fixed incident angle. Glycerol gradient solution (0%, 1%, 2%, 3% (weight percentage) glycerol dissolved in water) experiments were conducted, which indicate that the best fixed angle location is the middle of the linear range of SPR absorption peak and the central area signals are more uniform than those of the border areas. The sensitivity differences of different areas of SPR images are studied, and an optimized algorithm is developed.

A facile surface plasmon resonance (SPR) chip is developed for small molecule determination and analysis. The SPR chip was prepared based on a self assembling principle, in which the modified bovine serum albumin (BSA) was directly self-assembled onto the bare gold surface. The surface morphology of the chip with the modified BSA was investigated by atomic force microscopy (AFM) and its optical properties were characterized. The surface binding capacity of the bare facile SPR chip with a uniform morphology is 8 times of that of the bare control SPR chip. Based on the experiments of immune reaction between cortisol antibody and cortisol derivative, the sensitivity of the facile SPR chip with the modified BSA is much higher than that of the control SPR chip with the un-modified BSA. The facile SPR chip has been successfully used to detect small molecules. The lowest detection limit is 5 ng/mL with a linear range of 5—100 ng/mL for cortisol analysis. The novel facile SPR chip can also be applied to detect other small molecules.

In this paper, a surface plasmon resonance imaging (SPRI) system for cell analysis is developed for obtaining the surface plasmon resonance (SPR) signal from the interactions between cells and different stimuli. The system is constructed with a red laser light source, a P-polarizer, a glass prism, a 5× objective lens, a charge coupled device (CCD) camera, a gold sensor chip, a polydimethylsiloxane (PDMS) reaction well and a mechanical scanning device. The system is applied to mapping living cells in response to stimuli by characterization of the refractive index (RI) changes. Cell responses to K⁺in KCl solutions with concentrations of 5 mmol/L, 20 mmol/L, 50 mmol/L and 100 mmol/L are collected, which indicates that the SPRI method can distinguish the concentration of the stimuli. Furthermore, cell responses to epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) are studied independently. The binding of EGF receptor (EGFR) and EGF is collected as the first signal, and the internal change in cells is recorded as the second signal. The cell response to VEGF is different from that to EGF, which indicates that the SPRI as a label-free, real-time, fast and quantitative method has a potential to distinguish the cell responses to different stimuli.

为了实现高阻值纳米薄膜材料的热电系数测量, 搭建了一套塞贝克系数测量系统。研究了该系统的温控精度和温差生成机制并测量了高阻值条件下微弱电压。首先, 建立了高真空度和带有多重电磁屏蔽的真空测试环境; 然后, 设计了高稳定度温差控制平台, 以便为测试样品提供可控温差; 同时根据高阻条件下的微弱电压的检测要求, 消除了检测通道的漏电流和分布电容的影响。最后, 提出了一种循环温差的测量方法, 用于有效去除分布电容引起的塞贝克电压长期漂移。采用该方法对高阻值的有机半导体材料进行了塞贝克系数的测定, 结果显示: 阻值高达7×1012Ω的有机薄膜材料的塞贝克系数的测量精密度<2%,温度控制精度为±0.001 K。得到的结果表明, 该系统能够实现对样品阻值高达1012Ω的纳米薄膜材料的塞贝克系数的测量。

研究了外部光反馈对980nm垂直腔面发射激光器(VCSEL)振荡特性的影响.计算了垂直腔面发射激光器及边发射半导体激光器的光反馈灵敏因子.基于复合腔理论,分析了外部光反馈对垂直腔面发射激光器的阈值电流及微分量子效率等振荡特性参数的影响.实验结果表明,当反馈率为10%时,垂直腔面发射激光器的阈值电流由0.63A下降至0.59A,同时斜率效率和输出功率也有所下降.实验结果和理论分析符合得较好.